Volume 1

The collected papers of Sydney Ringer.

  • Ringer, Sydney, 1835-1910.
Date:
1912
Catalogue details

Licence: Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)

Credit: The collected papers of Sydney Ringer. Source: Wellcome Collection.

Provider: This material has been provided by Royal College of Physicians, London. The original may be consulted at Royal College of Physicians, London.

    462/538 (page 422)
    172 S. RINGER AND II. SAINSBURY. The following experiments were made:— Experiment I. Oct. 18. Serum from sheep’s blood shed on Oct. 15, diluted by the addition of two volumes of distilled water. Of this fluid lOc.c. were placed in each of six test tubes. One test tube was used as control to the other five, the following quantities of a lO^/o solution of calcium chloride were added, viz., 1, 2, 4, 6 and 12 drops respectively. In the results these numbers will represent the several test tubes. The test tubes were then placed in a water-bath the temperature of which was gradually raised. At 58C. 61 „ 63 „ 65 „ 69 „ 74 „ 78 „ 85 91 „ 4 and 6 became slightly turbid. They were markedly turbid. 12 was distinctly turbid. 4, 6, 12 were completely opaque. 2 was opalescent. 4, 6, 12 were semisolid. 2 more opaque, 1 slightly turbid as compared with the control. 2 nearly as opaque as 4, 6, 12 but much more fluid. 1 distinctly opaque. 4, 6, 12 more solid; 2 semisolid; 1 more opaque. Control slightly turbid. 4, 6, 12 solid, firm; 2 solid; 1 opaque and thick; control opal- escent. 1 had set; control more opalescent but quite fluid. The order of separation in this experiment was therefore in descend- ing sequence, beginning with the higher percentages of calcium chloride solution. The test tubes 4 and 6 came out a little earlier than the 12 ; this however was probably accidental as the difference was but slight. [In this experiment the drops were from the bottle and were larger than those of preceding experiments. In all subsequent experiments the drops were from a pipette and were uniform in size. They were smaller than the above.] Experiment II. In another experiment made on Oct. 18, with a nitrate of lime solution 10/^. The quantities of Experiment I. were repeated. The results obtained were as follows :— 65 C. 4, 6, 12 turbidity commencing, but most marked in 12. 70,, 12 quite opaque; 4 and 6 turbid. 71 „ 12 densely opaque, semisolid; 6 more opaque than 4. 75,, 6 very opaque, 4 less so ; 2 faintly opaque. 78,, 4 quite opaque but less than 6 ; 2 distinct opacity. 79 „ 6 semisolid; 4 thick.