The mechanism of hæmolysis with special reference to the relations of electrolytes to cells / by G.N. Stewart.

  • George Neil Stewart
Date:
[1909?]
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Licence: Public Domain Mark

Credit: The mechanism of hæmolysis with special reference to the relations of electrolytes to cells / by G.N. Stewart. Source: Wellcome Collection.

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    permeability of artificial lipoid membranes for proferments observed ° by Swart® or there may be adsorption, without splitting, of lipoid.® Arrhenius’ explains agglutination by acids as due to coagulation of proteins in the envelope... The entrance of acids into the corpuscles and therefore their hemolyzing power is accelerated by lecithin. But he gives no satisfactory explanation of how this is brought about. Baumgarten® restates his “osmological” explanation of hemolysis. He finds the fundamental cause of the liberation of the blood-pigment in an altered permeability of the stroma. He correctly distinguishes between total destruction of the corpuscle and liberation of the hzemo- globin. Up to a certain point his ideas coincide with those expressed in my first paper ten years ago, and repeated and emphasized in several succeeding ones. In essential points also my long-published views do not seem to differ fundamentally from some of those more recently put forward by Koeppe, Liebermann, and K. Meyer. Meyer® examined the mechanism of saponin hemolysis. He states that the first experi- ments on the mechanism of saponin-laking were undertaken by Ransom’® Two years earlier I published experiments on this subject, in which by physico-chemical methods it was demonstrated that a change in the per- meability of the corpuscles to electrolytes and water is an essential part of the saponin action. Later’ it was shown that the action on the per- meability of the corpuscles as regards ions still took place when they had been fixed by formaldehyde, and that the constituent on which the saponin acted might therefore be assumed to be non-protein. Ran- som’s valuable investigation concerns the constituent attacked. He says it is cholesterin. My experiments concern the immediate physico- chemical results of the attack and the way in which those physico- chemical changes lead to the liberation of blood-pigment. Meyer™ denies that Ransom’s experiments prove that cholesterin is the point of attack. He concludes, himself, that the action of saponin on the lecithin of the corpuscles is the important thing.“ > Bio-chem. Zeit., vi, p. 358, 1907. ® Dauwe: Hofmeister’s Beitr. vi, p. 426, 1905. 7 Bio-chem. Zeit., xi, p. 161, 1908. * Bio-chem. Zeit., xi, p. 21, 1908. ® Hofmeister’s Beitr. xi, p. 357, 1908. 10 Deutsch. med. Woch.. xxvii, p. 194, 1901. 11 Journ, of Physiol. xxiv, p. 224, 1899. 22 Tbid., xxvi, p. 470, 1901. 18 Archiv fiir Hygiene, Ixv, p. 293, 1908. 44 Cf. Pascucci: Hofmeister’s Beitr. vi, p. 543, 1905, and Porges and Neubauer: Wien. klin. Woch., p. 1285, 1907; Bio-chem. Zeit., vii, p. 152, 1908.
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